Journal: Acta Veterinaria Scandinavica

Article Title: Agreement between commercial assays for haptoglobin and serum amyloid A in goats

doi: 10.1186/s13028-017-0333-9

Figure Lengend Snippet: A line of equality plot and a Bland–Altman plot for 147 measurements of haptoglobin (Hp) obtained using a colorimetric assay (CA) and a competitive ELISA (cELISA) in goats. LoA stands for 95% limits of agreement

Article Snippet: The SAA concentration was determined in each serum sample also by using two commercial tests: (1) a solid phase sandwich ELISA (SAA-sELISA) (PHASETM RANGE multispecies SAA ELISA kit, Tridelta Development Ltd., Ireland;) and (2) a SAA-cELISA (Goat serum amyloid A SAA ELISA kit, Cusabio Wuhan Huamei Biotech Co., Ltd., China;).

Techniques: Colorimetric Assay, Competitive ELISA

Journal: Acta Veterinaria Scandinavica

Article Title: Agreement between commercial assays for haptoglobin and serum amyloid A in goats

doi: 10.1186/s13028-017-0333-9

Figure Lengend Snippet: A line of equality plot and Bland–Altman plot for 138 measurements of serum amyloid A (SAA) obtained using a sandwich ELISA (sELISA) and a competitive ELISA (cELISA) in goats. LoA stands for 95% limits of agreement

Article Snippet: The SAA concentration was determined in each serum sample also by using two commercial tests: (1) a solid phase sandwich ELISA (SAA-sELISA) (PHASETM RANGE multispecies SAA ELISA kit, Tridelta Development Ltd., Ireland;) and (2) a SAA-cELISA (Goat serum amyloid A SAA ELISA kit, Cusabio Wuhan Huamei Biotech Co., Ltd., China;).

Techniques: Sandwich ELISA, Competitive ELISA

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Primer sequences used for quantitative PCR

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques:

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Radiation time response in mouse SAA1 measured using ELISA in 8 Gy irradiated female C57BL/6J mice at 0, 1, 2, 4, and 6 hours post-irradiation. n=3 per group. *, P<0.05 in the irradiated mice compared with control mice.

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Time and dose response of SAA1 after TBI. (A) SAA1 was measured using ELISA in 0, 1, 2, 4, 8 and 12 Gy irradiated female C57BL/6J mice at 0.125, 0.5, 1, 2, 3, 5 and 7 days post-irradiation. SAA1 dose-dependent change at (B) 0.25, (C) 0.5, (D) 1, (E) 2, (F) 3, (G) 5 and (H) 7 days after exposure to radiation. Each black dot represents one animal. Error bars indicate ± 1 SD for each radiation exposure group. n=8 per group (n=7 at 7 days after 8 Gy, n=2 at 7 days after 12 Gy). SAA1, serum amyloid A1; TBI, total body irradiation.

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: P values associated with two-tailed Student’s t -test for SAA1 concentrations after comparing control and irradiated groups

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: SAA1 mRNA time-dependent increase after radiation in (A) liver, (B) lung, (C) thymus, (D) spleen, (E) bone marrow and (F) small intestine measured using Quantitative PCR in control and 8 Gy irradiated female C57BL/6J mice at 0.125, 0.5, 1, 2, 3, 5 and 7 days post-irradiation. Error bars indicate ± 1 SD for each radiation exposure group. n=6 to 8 per group. *, P<0.05, *, P<0.01, ***, P<0.001, and ****, P<0.0001 in the irradiated mice compared with the control mice. SAA1, serum amyloid A1.

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Real-time Polymerase Chain Reaction, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: SAA1 expression and systemic infection parameters in the same mice. (A) SAA1 and LPS in serum and 16S rRNA in the liver of the same animals measured on 31 mice on day 0,1, 5 and 7 (7 mice on day 7, and 8 per group on day 0, 1 and 5) after exposure to 8 Gy TBI. (B) SAA1 and PCT in serum in the same animals was on 23 mice on days 0, 0.125, 0.5, 1, 2, 3, 5 and 7 (2 mice on days 7 and 3 per group on days 0, 0.125, 0.5, 1, 2, 3 and 5) after exposure to 12 Gy TBI. SAA1, serum amyloid A1; TBI, total body irradiation.

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Expressing, Infection, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Multiple linear regression analysis between serum SAA1 concentration and number of lymphocyte and irradiation dose

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Concentration Assay, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: SAA1 cut-off values of classification within 2 days post-irradiation

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques:

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: PBI patterns and SAA1 response. (A) The liver of C57BL/6J mice was shielded in PBI-1 and PBI-3 and in the corresponding groups PBI-2 and PBI-4 the liver was exposed. (B) SAA1 concentration in serum and (C) mRNA expression in liver of control, total-body irradiation and PBI group at 12 hours after 8 Gy irradiation. Error bars indicate ±1 SD for each radiation exposure group. n=6 per group. **, P<0.01, ***, P<0.001, and ****, P<0.0001 in the irradiated mice compared with the control mice. PBI, partial body irradiation; SAA1, serum amyloid A1.

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Concentration Assay, Expressing, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Dynamic SAA1 concentration and corresponding date of death within 30 days after 10 Gy irradiation

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Concentration Assay, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Mean and standard deviation (SD) of SAA1 concentration in 92 healthy mice

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Standard Deviation, Concentration Assay

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Kaplan-Meier survival curves of mice. P value determined by log-rank test. Serum SAA1 in mice treated with amifostine before 10 Gy irradiation and in the 10 Gy group on day −4, 1, 3, 5 and 7. n=8 in 10 Gy + Amifostine group, n=20 in 10 Gy group. ***, P<0.001. SAA1, serum amyloid A1.

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: SAA1 concentration in the Amifostine group and corresponding death date within 30 days

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Concentration Assay, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Clinical parameters and corresponding SAA1 concentration in all 17 NPC patients

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Concentration Assay

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Effect of radiotherapy on serum SAA1 in nasopharyngeal carcinoma patients. (A) The scatter plot shows SAA1 concentration before radiotherapy and corresponding expression after radiotherapy in 17 patients with nasopharyngeal carcinoma (****, P<0.0001). (B) ROC curve of SAA1 as a biomarker for predicting radiation exposure in patients with nasopharyngeal carcinoma. SAA1, serum amyloid A1.

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Concentration Assay, Expressing, Biomarker Assay